Other

Part:BBa_K779500:Design

Designed by: Kristjan Eerik Kaseniit   Group: iGEM12_MIT   (2012-09-29)

Long reporter top strand with bulge MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

4-nucleotide bulge added to Part:BBa_K779501. Bulge placed 10-nt from the 3' end as a potential protection mechanism from cleavage by RISC as suggested by [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333889/ Iba et al. 2011]. See Part:BBa K779501 for sequence design besides the bulge.

Source

De novo design.

References